In Vitro Induction of Shoots of Chickpea (Cicer Arietinum L.) Using Ms Medium under Aseptic Conditions

Abstract This study investigates the in vitro induction of shoots in chickpea (Cicer arietinum L.) using Murashige and Skoog (MS) medium under aseptic conditions in a laminar airflow chamber. Chickpea is an essential legume with high nutritional value, serving as a vital source of protein, fiber, and essential nutrients. The aseptic in vitro culture technique aims to enhance shoot induction for mass propagation and genetic improvement. Seeds were disinfected and inoculated onto Murashige and Skoog (MS) medium supplemented with different concentrations of IAA Indole Acetic Acid (0 mg/L, 0.3 mg/L, and 0.6 mg/L). Arya, (S. Rathi et al., 2020). Observations recorded between 3 to 9 days post-inoculation revealed that the highest IAA concentration (0.6 mg/L) resulted in the best shoot induction. These results highlight the critical role of IAA in shoot morphogenesis, demonstrating that 0.6 mg/L is the optimal concentration for shoot initiation and elongation in Cicer arietinum L.( Rashid, H et al., 2015). This study contributes to the understanding of Auxin-mediated shoot regeneration in legumes, offering insights for future plant tissue culture applications. The study demonstrates the effectiveness of MS medium and optimal culture conditions for shoot initiation.