PHARMACOLOGICAL EVAUTIONS OF METHANOLIC EXTRACT AND FRATIONS OF BUTEA MONOSPERMA

The i.c.v injection of Aβ peptide in negative control animals showed an extremely significant (P<0.001) increase in brain AChE levels. The AChE enzyme levels were significantly decreased in the treatment groups and indicated a difference with p<0.01, p<0.05, p<0.001 and p<0.05, respectively when compared with the amnesia induced group. The levels of each animal expressed in µmole/min/g. From the results, it was found that acetyl cholinesterase levels are increased in negative (0.23±0.015) when compared to control (0.10±0.0012), and decreased levels are observed in methanol extract (0.15±0.0026 and 0.13 ±0.0031 for 200 and 400 mg/kg, respectively) (Figure 4.8). The AChE enzyme levels were significantly decreased in the treatment groups of BME (0.15 ± 0.0026 and 0.12 ± 0.0023), BMC (0.19 ± 0.0025 and 0.16± 0.007), BMH (0.17 ± 0.0021 and 0.13 ± 0.0018) and BMQ (0.21 ± 0.0046 and 0.18± 0.0051) and indicated a difference with p<0.01, p<0.05, p<0.001 and p<0.05. Glutathione levels are decreased in negative (0.0159±0.00023) when compared to control (0.0298±0.00014), and levels are increased in methanolic extract of 200 mg/kg and 400 mg/kg respictively as follows 0.0266±0.00186 and 0.0275± 0.00 14. Glutathione levels are decreased in negative (0.0159±0.00023) when compared to control (0.0298±0.00014), and levels are increased in BME (0.0256±0.0018 and 0.0279 ± 0.0025), BMC (0.0239±0.0013 and 0.0253±0.0027), BMH (0.0247 ± 0.0008 and 0.0268 ± 0.0004) and BMQ (0.0227±0.0011 and 0.0242±0.0024) fractions.