In Vitro Assessment of Cytoprotective Activity of Buchanania Lanzan (Chironji Seeds)

Ayurvedic and Unani systems have long utilized Buchanania lanzan, often known as chironji, as a medicinal herb because of its therapeutic qualities. Using an in vitro MTT test, this study assesses the cytoprotective efficacy of ethanolic extracts of chironji seeds on oxidatively stressed L929 fibroblast cells. While co-treatment with the seed extract sustained cell viability in a dose-dependent way, hydrogen peroxide-treated cells displayed decreased viability. Viability was high at 84.88% at 100 µg/mL, whereas it was 99.09% at 20 µg/mL. These findings show that B. lanzan extract can reduce oxidative stress-induced cellular damage, indicating that it may be used in formulations to treat conditions linked to oxidative stress. Using the MTT test, cytoprotective efficacy was evaluated in L929 fibroblast cells exposed to oxidative stress. Protective actions against cellular stress were demonstrated by the extract's enhancement of cell viability and mitigation of oxidative damage. These components are known to play a role in the cytoprotective mechanisms that the study found. They may help fight diseases linked to oxidative stress if they are added to nutraceuticals or medicinal formulations. This study not only supports conventional wisdom but also offers empirical evidence for the therapeutic benefits of chironji seeds in contemporary medical settings.