Cytotoxic Test Extract Ethanol Black Sea Cucumber ( Holothuria atra ) Against Line Cells HSC -3 in General In Vitro

Oral Squamous Cell Carcinoma (OSCC) is one of the most aggressive malignant tumors due to its tendency to spread and high recurrence rate. Sea cucumbers have compounds that show anti- microbial, anti- inflammatory, and anticancer effects. The aim of this study was to determine the anticancer activity extract ethanol black sea cucumber ( Holothuria) atra ) against HSC-3 cell line in vitro. Extract black sea cucumber ( Holothuria) atra ) is done with the type of extraction maceration, then continued with u test Cytotoxicity by MTT Assay method using extract ethanol black sea cucumber ( Holothuria) atra ) at concentrations of 240 μg / ml, 120 μg / ml, 100 μg / ml, 80 μg / ml, 40 μg / ml, 20 μg / ml, 10 μg / ml, 5 μg / ml. At a concentration of 240 μg / ml has a cell viability of 35.215%, doxorubicin has a cell viability of 66.518%. The conclusion of this study is that there is cytotoxicity of ethanol extract of black sea cucumber ( Holothuria atra ) against HSC-3 cell line with IC50 value obtained of 41,371 μ g/ml using ethanol extract of Holothuria atra with concentration of 240 μ g/ml, 120 μ g/ml, 100 μ g/ml, 80 μ g/ml, 40 μ g/ml, 20 μ g/ml, 10 μ g/ml, 5 μ g/ml. At a concentration of 240 μg /ml there is a cell viability of 35,215%, this value is in the moderate toxicity category compared to the positive control of doxorubicin 0.1 μg /ml with a cell viability of 66,518% so that the extract ethanol black sea cucumber (Holothuria) atra ) has the potential to be an alternative to anticancer drugs.