Aspistomella duo Kovac, Kameneva & V. Korneyev 2024, sp. nov.

Aspistomella duo Kovac, Kameneva & V. Korneyev, sp. nov. urn:lsid:zoobank.org:act: 69AD65D5-477E-4DA8-AC63-6050B37FFEFF Figs 1 B, 5 C, 7 A, 9 A, G, 14–21 Material. Type. Holotype ♂: “ Bolivia: Buena Vista, near Santa Cruz [El Cairo, 17.5009S, 63.6743W]; on upright bamboo culm (Guadua chacoensis, last year); 29.i.2011; Bol 91/11 leg. D. Kovac ” (SMF). Paratypes: 2♂, 1♀ (1♂, 1♀ dissected); Bolivia: same data as for holotype (D. Kovac leg.) (SIZK); 2♀, same data as for holotype (D. Kovac leg.); larva in bamboo internode (Guadua chacoensis, last year), emerging on 8.ii.2011, Bol 156/111, (leg. D. Kovac) (SMF). Non-type. Bolivia: Buena Vista, near Santa Cruz (El Cairo); [17.4734S, 63.6922W], 1.xii.2007, Bol.76.07; 1 ♂ (alcohol collection) (D. Kovac leg.) (SMF); 1.12.2007, Bol.77.07, 5♂; (alcohol collection) (SMF); same label, 1♂, (SEM stub) (SMF); same label, 1♂ (SIZK). Larvae. Bolivia: Buena Vista, 6.ii.2011, BolZ32/11, 6 larvae (D. Kovac leg.) (SMF). Diagnosis. Aspistomella duo is similar to other species of the genus having a very short pterostigma, shorter than the width of the costal cell (Fig. 5 C), but differs from them in details of the wing pattern: apical quarter of cell r 4+5 completely dark (in A. heteroptera, A. enderleini, A. lobioptera, A. lunata, A. steyskali, crossed by crescent hyaline incision); Aspistomella duo is similar to A. tres in having wing apex without hyaline incisions crossing cell r 4+5 and vein R 1 dorsoapically setulose, but differs from it in having cell dm between crossveins r-m and dm-m completely dark and a conspicuously narrower wing. Description. Adult. Male. Head (Figs 14 B–D) ratio (length: height: width) = 1: 1.4: 1.75, frons, parafacial and gena reddish or yellowish brown, except anterior part of frons. Lunule and face between antennae yellow. Occiput mostly black, at least in dorso-lateral part. Frons (Fig. 14 D, C, 15 A) 1.15× longer than wide, with shiny, partly brown or black ocellar triangle and brown vertical plates; orbits silver-white microtrichose; parafacial narrow, 0.2–0.3× broader than postpedicel, white microtrichose. Frontal setulae short, moderately strong, black, proclinate and partly inclined, forming three irregular rows of 2–6 setulae on each side, anteriormost setulae longer than others and as long as outer vertical seta; 1 very long orbital seta, 1.3× longer than ocellar seta, 0.75× longer than inner vertical seta and 2.5× longer than outer vertical seta; postocellar seta long, 0.4× longer than inner vertical seta; outer vertical seta short, 0.3–0.4× longer than inner vertical seta and 2–3× longer than postocular setae forming one row, allied by 8–9 dorsal and 13–19 ventral occipital setae. Lunule very narrow, indistinct, orange. Eye 1.5–1.7× higher than long. Face (above suture) 0.5× higher than wide at transverse fold, yellow to brownish yellow, densely white microtrichose; epistome reddish brown to black (medially), sparsely white microtrichose, with metallic greenish or golden sheen, strongly produced anteriorly and separated from upper part of face by deep suture. Clypeus yellow, densely white microtrichose, matt, 0.5–0.6× higher than epistome. Gena brownish yellow, with long genal seta and 5–6 additional setae anterior to it, 0.6–0.8× longer than genal seta. Occiput black, except postgena brownish-yellow, with yellowish to cyan metallic sheen, moderately densely covered with greyish microtrichia partly hiding underlying cuticle; ventral part of occiput (postgena) with 4–6 setae almost as long as genal seta. Antenna brownish yellow; scape and pedicel with black setulae; postpedicel brown, often blackish apically, whitish microtrichose, 2–2.2× longer than wide, apically rounded; arista black except yellow at base, almost bare. Mouthparts black, prementum black, shiny. Palp yellow, narrowly crescent-shaped, apically rounded, with 10–12 strong black setulae, of which 2–3 subapical setulae 1.5–2× longer than other setulae. Thorax (Figs 14 E, 16 A) brown to black, with bluish sheen and sparse white microtrichia not obscuring underlying cuticle. Mesonotal scutum 1.3× longer than wide; presutural area with large black patch and brown margin, including postpronotal lobes and notopleuron; 3 pairs of black vittae: short submedially, reaching posteriorly only to level of postsutural supra-alar seta, intra-alar and supra-alar vittae fused anteriorly at transverse fold and reaching level of intra-alar setae and yellow to dark brown prescutellar area; black setulose, with 10–14 rows of setulae between rows of dorsocentral setulae; acrostichal prescutellar setae present (in 3 of 6 specimens examined), absent (in 2 of 6 specimens examined), or present on one side only (in 1 specimen examined); prescutellar area medially setulose and without setulae around dorsocentral setae. Scutellum dorsally very slightly convex, orange to reddish brown, matt, sparsely microtrichose, without setulae, laterally sometimes with inconspicuous silvery sheen. Subscutellum subshining, dark brown. Mediotergite shiny black, sparsely microtrichose. Other setae as described for the genus. All the setae and setulae black. Wing (Fig. 5 С) 6.1–7.7 mm (♂), 5.7–7 mm (♀) long, 2.9–3.5× longer than wide; basicostal cell hyaline; costal cell lobed, 4× longer than wide, brown in basal and apical quarters of length, with costa conspicuously curved posteriorly and covered with slightly longer setulae before apex of vein Sc; pterostigma entirely brown, narrowly triangular, 0.4–0.5× longer than wide, vein R 1 with 3–4 setulae dorsally, ending slightly proximal of crossvein dm-m level in both sexes (Fig. 14 G); vein R 2+3 almost straight, subparallel to costa to very apex. Crossvein r-m slightly distal to center of cell dm in both sexes. Cell r 4+5 7–8× longer than wide, apically narrowed. Cell m 1 narrowly triangular; ultimate section of M 1 4–5× longer than crossvein dm-m and 2–2.5× longer than penultimate section. Vein CuA Z-shaped, cell cua with moderately short posteroapical lobe along vein CuP. Wing pattern with short subbasal crossband from humeral vein to base of cell cua and large dark brown, Z- or 2-shaped mark widely from apical lobe of costal cell almost to base of cell dm and longitudinal fold of cell m 4, and 2 wide triangular incisions from cell r 1 into cell dm distally from crossvein r-m and from base of cell m 1 into cell r 4+5. Calypters white, with white ciliae. Halter brown with black or brown knob (Figs 14 H, 16 G, H). Legs (Figs 14 A, F, 16 I–O) with yellow or brownish-yellow coxae and trochanters, femora entirely yellow to entirely brown; tibiae and tarsi either brownish yellow or brown, in 2 specimens contrasting with yellow femora, black setose and setulose, without conspicuous microtrichia or metallic sheen in examined specimens. Fore femur with 2 rows of posterodorsal and posterior setae, and 3–4 preapical posteroventral setae, all slightly shorter than femur width. Mid femur anteriorly and posteriorly with short setulae, but without long erect setae; mid tibia ventrally with two subequal spur-like setae 2–2.5× longer than tibia width (Fig. 16 K). Tarsi as on Figs 17 J–O. Abdomen moderately elongate (Fig. 16 C) brown to completely black, syntergite 1+2 often brownish-yellow; tergites finely sparsely microtrichose, with faint cyan metallic sheen, except tergite 5 of ♂ brown or black without metallic sheen; setulae and setae black. Male tergites 3, 4 and 5 of subequal length. Female tergite 6 short, hidden underneath tergite 5; sternite 6 transverse; oviscape as long as tergites 4–6 combined. Postabdomen. Cerci short setulose, dorso-ventrally flattened and broadly fused, posteriorly very shallowly bilobate (Figs 17 D, E). Epandrium short setulose, with setulae 3–6 × narrower than base of surstylus (Figs 17 A, B). Each surstylus basally broad, gradually curved mesally, with a row of five shortened setulae on its mesal surface, two of which conspicuously thickened, but not strongly sclerotised (Fig. 17 A). Pregonites symmetrical, slightly lobed, with 5 setulae ventro-mesally, conspicuously anterior to postgonites (Figs 17 F, G, I). Postgonites rounded, with 5–6 small trichoid sensilla (Figs 17 F, H, I). Phallus basally short microtrichose, apically with numerous lamellar cuticular sclerotisation, about 3.5 mm long; in rest, coiled on right side of abdomen (Fig. 17 K). Female: all taeniae 0.66× longer than eversible membrane (Fig. 18 B); aculeus not flattened (Fig. 18 C), with long setulose ovoid cercal unit and (Fig. 18 C), 1.9 mm long; 2 subglobose spermathecae (Fig. 18 A, E). Third instar larva. General structure. Mature larva whitish, muscidiform, length 7.5–11.2 mm (median: 8.45 mm; n = 8), width 1.8–2.5 mm (median: 2.4 mm; n = 8), anteriorly conical, posteriorly rounded, broadest at caudal segment (or sometimes posterior end of abdominal segment VII), surface largely smooth except of spinules of the locomotory creeping welts on ventral abdominal segments I–VII and caudal segment, anterior spiracles fan-shaped, roundish spiracular discs dark, bearing three spiracular openings radiating in a sinuous line out from ecdysial scar. Pseudocephalon (Figs 19 A–F) flat ventrally, rounded dorsally, deep incision between cephalic lobes, ventral half of incision connected to oral cavity (Fig. 19 A). Antenna three-segmented, located dorso-laterally on cephalic lobe near head apex; basal segment broad and round, second segment slightly conical when extended and surface covered by two finger-like extensions of the epidermis, apical segment conical with rounded apex (often collapsed in SEM specimens) (Figs 19 A–C, E). Maxillary sense organs located on ventral side of cephalic lobes, maxillary palpus contains three papillary sensilla and two knob sensilla enclosed by an incomplete cuticular fold, dorsolateral group of sensilla contains two papillary sensilla (Fig. 19 F). Mandibles completely smooth with flattened apices, separated from each other by two median oral lobes (Fig. 19 C). Facial