Study of Thyroid Cytopathology With Use of Ultrasound Guided FNA and Modified Ultrafast Papanicolaou Staining in Increasing Diagnostic Accuracy

Abstract FNAC is a cheap and quick procedure which is also highly sensitive and specific in the categorization of thyroid lesions. It is a reliable, simple and cost-effective technique with minimal complications. The ever increasing use of FNAC as one of the pivotal diagnostic tools has validated the use of other stains like Romanowsky and haematoxylin and eosin (H&E) along with the conventional PAP stain. The standard protocol for PAP staining technique requires about 40 minutes. To resolve this issue in 1994, Yang and Alvarez invented the ultrafast Pap (UFP) stain.1 It is a hybrid of PAP stain and Romanowsky stain. It reduces the time of staining to 90 seconds with enhances the quality. Kamal et al2 invented the Modified Ultra-Fast Pap (MUFP) stains reagents required for the UFP stain were not universally available. They used easily available Gills or Harris hematoxylin instead of Richard Allan hematoxylin and modified EA instead of Richard Allan cytostain. MUFP stain results is exquisite cytomorphology in the form of large well spread and transparent cytoplasm; open and crisp chromatin on a background of clear nuclear sap and clean blood free background. Observational cross sectional study was carried out at tertiary care center where all FNAC samples of thyroid nodules were included. Out of that 150 samples were studied. Staining quality was assessed for parameters like smear-background, overall staining, nuclear, and cytoplasmic staining. thyroid carcinoma In our study we tried MUFP stain on intraoperative scrape smears of papillary, it helped in intraoperative diagnosis because of clear staining and reduced staining time Most studies using MUFP stain have been done malignant lesions particularly in thyroid and breast.3 The use of MUFP stain in evaluation of thyroid nodules can prove helpful to reduce the false negative rate. Quick diagnosis of FNAC plays an important role in efficient medical practices.