Spectrophotometric Method for The Determination of Lamivudine in Pharmaceutical Preparations

A simple and sensitive visible spectrophotometric method has been developed for the estimation of lamivudine in pharmaceutical preparations. The method is based on the reaction of lamivudine with 2, 3-dichloro-5, 6-dicyano-1, 4-benzquinone to form an red colour charge–transfer complex. The red coloured solution was measured at 450 nm against reagent blank. Lamivudine obeys Beer’s law in the concentration range of 20-100 μg/ml.