A Comprehensive Review of Methods for Testing in-vitro Anthelmintic Activity

Antihelmintic drug affect the human body and its normal functions by causing helminthiasis, a serious parasitic infection. Most parasites affecting humans and livestock develop resistance to these drugs, leading to increased morbidity and mortality.[1] The impact of parasitic worm infections on vulnerable hosts is a major concern, prompting research into the infection, prevalence, host-parasite interactions, and the parasite's resistance to treatment. This review highlights the rising prevalence of parasitic worm infections, which is linked to factors such as population growth, climate change, low living standards, particularly in conflict zones, and in developing countries.[2] This review aims to classify different species of anthelmintic plants based on their in vitro effectiveness. A range of plant species known for their anthelmintic activity were selected for evaluation. In vitro assays, including the paralysis time, death time, egg hatch test, larval migration assay, larval development test, and adult motility assay were included to measure the anthelmintic potential of the plant. Also focused on the use of plants and secondary metabolites against intestinal parasites. We discuss the use of plants in traditional medicine and the use of plant secondary metabolites tried in in vitro test.