Development And Validation of Novel Rp-HPLC Analytical Methods for The Simultaneous Estimation of Omeprazole and Aspirin in Bulk Drug and Pharmaceutical Formulation

A RP-HPLC method was developed and validated using Enable C18 250 x 4.6 mm, 5 µm column with mobile phase composition of Acetonitrile and Water in the ratio of 60:40 v/v, pH is adjusted to 2.9 by using Ortho-Phosphoric acid flow rate of 1.0 mL/min and UV detection at 240.0 nm, linearity was observed over concentration range of 20-100 µg/mL for omeprazole and 25-125 µg/mL for aspirin. The accuracy of the proposed method was determined by recovery studies and found to be of 99.09% to 100.12% for omeprazole and 99.6% to 100.12% for aspirin respectively. The proposed method was extended for estimation of omeprazole and aspirin in marketed tablet formulation and assay values conformed to the label claim of the marketed tablet formulation. This developed and validated HPLC method can be used for routine analysis of omeprazole and aspirin in tablets.