Design Of Experiment Approach for Development of Microspheres Loaded Gel of Nyctanthes Arbor Tristis Leaves Extract for Enhances Stability as Well as Bioavailability of Phytoconstituents

Qualitative phyto chemical screening of Nyctanthes arbor tristis showed the presence of active metabolites such as Alkaloids, carbohydrates, glycosides, tannin and phenols is present. Quantitative phytochemical assay was performed by calculating total phenolic content (TPC) and total flavonoid content (TFC). Microspheres were prepared by the chemical denaturation method using glutaraldehyde as a cross-linking agent. The microspheres were characterized by particle size analysis, zeta potential, scanning electron microscopy (SEM) and stability study. Several factors such as stirring rate, temperature, and viscosity show an effect on the size. The sizes of the microspheres were found to be 156.5 nm. The current study involved the formulation and characterization of extract-loaded microspheres. SEM photographs confirmed the shape and formation of the microspheres. These microspheres are employed in the delivery of drugs and extracts; the drugs may be encapsulated or entrapped. In future it can also be formulated in various dosage forms.