Synergistic Detection, Quantification and Standardization of Oral Dental Preparation Comprising Curcumin and Eugenol By RP-HPLC Method

In the current study, a reverse phase high performance liquid chromatographic method has been developed and validated for standardization of dental preparation on the basis of Curcumin and Eugenol. Both the active components were determined and quantified using Agilent 1260 Infinity II, with the mobile phase consisted of 40 parts acetonitrile, 30 parts methanol, and 30 parts water. The separation was performed using a Supphirus C18 HP Classic Column (250*4.6 mm, 5 µm). In order to obtain the chromatogram, 240 nm was set as analytical wavelength, the flow rate was kept constant at 1 ml/min. At 4.692 and 5.985 minutes, respectively, the chromatogram showed the elution of Curcumin and Eugenol. Linearity showed up for both Eugenol and Curcumin comprising the range of 0.5 to 5 µg/mL. For Curcumin and Eugenol, the values of R2 were determined to be 0.996 and 0.994, respectively. It was discovered that the LOD for Eugenol and Curcumin was 0.01 and 0.03 µg/mL, respectively. For Eugenol and Curcumin, the LOQ was found to be 0.03 and 0.11 µg/mL, respectively. The ICH Q2 (R2) guideline was successfully followed in the validation of the optimized method.