APPROBATION OF METHODS FOR FLUORIMETRIC DETERMINATION OF PHOTOSYNTHETIC ACTIVITY OF PHYTOPLANKTON CELLS

Various FRR protocols were tested for registration of fluorescence induction curves of phytoplankton chlorophyll on a laboratory test culture Chlorella sorokiniana, as well as on cells of natural phytoplankton from the Kalmius and Krynka rivers. The results of successful field tests on the registration of fluorescence induction curves at the intensity of saturating pulses of 20, 30 and 50 thousand μmol.·quanta·m-2·s-1 with a duration of 1 μs with different time intervals: 1, 4, 9, 19, 29 and 49 μs with a total measurement duration of 1 second. It is shown that FRR protocols with pulse intervals of 1, 4 and 9 μs are the most suitable for measuring fluorescence in natural conditions.