Feed Forward Loop Improves the Transient Dynamics of an Antithetic Biological Controller

The ODE-based models were constructed for each antithetic controller design and solved utilizing the MATLAB ode23s solver. These models accounted for species interactions involving activation, degradation, sequestration, and repression, with kinetic parameters derived from established literature in the field. The sensitivity analysis was executed by systematically altering all kinetic parameters within the model. In each simulation, the designated kinetic parameters for each circuit were multiplied by a "scaling factor," spanning from 10-1 to 10.