Studies on the Evaluation of Flavonoids (Morin, Naringin, Quercetin & Rutin) in Psidium Guajava Linn. Leaves By HPLC

Flavonoids are polyphenolic compounds recognized for their antioxidant properties and numerous health benefits. This study aimed to evaluate and quantify the flavonoids Morin, Naringin, Quercetin, and Rutin in Psidium guajava Linn leaves. High-Performance Liquid Chromatography (HPLC) was used for the qualitative analysis of these compounds, utilizing a reverse-phase column with a mobile phase consisting of 0.1% phosphoric acid and acetonitrile in a 25:75 ratio. The standard retention times for Quercetin, Morin, Naringin, and Rutin were 2.513 min, 3.887 min, 7.130 min, and 4.780 min, respectively. In the *Psidium guajava* Linn leaf extract, Naringin and Quercetin were detected at retention times of 7.13 min and 2.527 min, respectively. These findings confirm the presence of significant flavonoids with potential health benefits in Psidium guajava Linn leaves.