Mideopsis roztoczensis Biesiadka & Kowalik 1979

Mideopsis roztoczensis Biesiadka & Kowalik, 1979 Fig. 8 Material examined. Portugal, Beja: • Mértola, Moinho de Alferes 1, 37.502°N, 7.69°W, 19 May 2023, leg. Ferreira, Benitez-Bosco, Ekrem, Stur & Turaccio, 1 ♀ (sequenced); Mértola, Pulo do Lobo, 37.805°N, 7.633°W, 18 May 2023, leg. Ferreira, Benitez-Bosco, Ekrem, Stur & Turaccio, 5 ♀, 1 deutonymph (sequenced); • Odemira, Ribeira de Seixe, Zambujeira do Mar, 37.398°N, 8.68°W, 75 m a.s.l., 23 May 2023, leg. Ekrem & Benitez-Bosco, 1 ♂ (sequenced). Bragança • Vinhais, Gasparona, 41.85°N, 7.013°W, 683 m a.s.l., 6 Jul. 2023, leg. Ferreira & Padilha, 1 ♀, 1 deutonymph (sequenced). Guarda: • Seia, Casa do Loureiro, 40.433°N, 7.701°W, 415 m a.s.l., 19 Jul. 2023 leg. Ferreira & Padilha, 1 ♂, 3 ♀, 1 deutonymph (sequenced); • Manteigas, Zêzere, Covão da Ametade, 40.328°N, 7.587°W, 1431 m a.s.l., 21 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 1 ♂, 1 ♀, 1 deutonymph (sequenced); • Manteigas, Zêzere, Ponte dos Frades, 40.403°N, 7.526°W, 672 m a.s.l., 22 Aug. 2023, leg. Ferreira, Benitez-Bosco, Padilha, Andrade & Stur 1 ♀ (sequenced); • Manteigas, Poço do Inferno, 40.373°N, 7.516°W, 1078 m a.s.l., 21 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 2 ♂, 1 ♀ (juv.), 1 deutonymph, 1 ♂ dissected and slide mounted (RMNH); • Gouveia, Rio Mondego, Casais de Folgosinho, 40.454°N, 7.493°W, 976 m a.s.l., 24 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 1 ♂, 1 ♀ (sequenced); • Manteigas, Poio do Leão, 40.399°N, 7.541°W, 734 m a.s.l., 22 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 2 ♂ (sequenced); • Seia, Rio Alva, Central hidroelétrica de Ponte dos Jugais, river, 40.385°N, 7.706°W, 555 m a.s.l., 23 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 1 ♂, 2 ♀, 2 deutonymph (sequenced), 1 ♂ dissected and slide mounted (RMNH); • Rio Alva, Nossa Senhora do Desterro, river, 40.395°N, 7.694°W, 791 m a.s.l., 23 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 2 ♀ (sequenced); • Manteigas, Mondego, Covão da ponte, 40.443°N, 7.514°W, 999 m a.s.l., 24 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 1 ♂ (sequenced); • Seia, Rio Alva, Praia Fluvial de Sabugueiro, river, 40.401°N, 7.64°W, 1021 m a.s.l., 24 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 2 ♀ (sequenced); • Manteigas, Casa do Cantoneiro, 40.418°N, 7.603°W, 1378 m a.s.l., 24 Aug. 2023, leg. Ferreira, Benitez-Bosco & Padilha, 2 ♂, 1 ♀ (sequenced). Vila Real • Murça, Noura stream, 41.409°N, 7.417°W, 421 m a.s.l., 12 Jul. 2023 leg. Ferreira S, Padilha 1 ♀ (sequenced). Remarks. The specimens examined in our study match the description of Mideopsis roztoczensis, when following Biesiadka and Kowalik (1979). Mideopsis roztoczensis is characterized by a more elevated dorsal shield with distinctly visible anteriorly diverging lines of particularly faint fine porosity, and by the the shape of the male ejaculatory complex with the wedge-shaped anterior ramus being wider and with a characteristic arrow-shaped delimited area. Recently, Pešić et al. (2023 e) showed that M. roztoczensis is a genetically variable species, comprising four BINs (BOLD: ACI 1492, BOLD: AEN 6785, BOLD: AEA 2936, BOLD: AEO 2944) widely distributed in Europe. In this study, we detected an additional five unique BINs (BOLD: AFU 6108, BOLD: AFP 5421, BOLD: AFW 3785, BOLD: AFV 6334, BOLD: AEV 2909) within the Portuguese M. roztoczensis - like specimens (Fig. 8), all of them unique, and some of them present at the same sites (e. g., BOLD: AFU 6108 and BOLD: AFW 3785 in Casa do Loureiro, BOLD: AFU 6108 and BOLD: AFV 6334 in Casa do Cantoneiro). Distribution. Europe, Turkey. New record for Portugal.