THE EFFECT OF 96% ETHANOL EXTRACT OF TURMERIC (Curcuma longa Linn. syn. Curcuma domestica Val.) AGAINST ESTROGEN HORMONE

Abstract Background: During a woman's reproductive period (puberty to pre-menopause) the structure and function of the reproductive organs are maintained by the hormone estrogen. When entering menopause, estrogen hormone levels drop, causing the symptoms of menopause. To overcome menopause symptoms, hormone replacement therapy (HRT) is widely used with HRT where the hormone estrogen has been proven to reduce menopause symptoms, although it can increase the risk of breast, endometrial, and ovarian cancer. With these risks from HRT, it is necessary to find alternatives to overcoming menopause symptoms. One alternative that is widely developed is the herbal ethanol extract of turmeric (Curcuma longa Linn. syn. Curcuma domestica Val.) Objectives: This study aims to analyze the effects of turmeric on estrogen hormones in rats. Methods: This study is an experimental study. The study was conducted for 30 days using 15 female Sprague-Dawley rats. The treatment consisted of 5 groups with a dose of 0 mg/Kg BB as a control, 10 mg/Kg BB Contraceptive Pills, a dose of 25 mg/Kg BB, a dose of 50 mg/Kg BB, a dose of 100 mg/Kg BB given orally via 1 ml of vial. On the 31st day, surgery and serum were taken from the rats. Estrogen hormone levels were tested using the Enzyme Linked Immunosorbent Assay (ELISA) method. Results: The average estrogen levels in all treatment groups were higher than the Control group with an average estrogen hormone ELISA result of 647 ng/L, P1 (given turmeric ethanol extract ( Curcuma longa Linn. syn. Curcuma domestica Val.) as much as 25 mg) from the results of the ELISA test of estrogen hormone, an average of 691 ng/L was obtained, P2 (given ethanol extract of turmeric ( Curcuma longa Linn. syn. Curcuma domestica Val.) as much as 50 mg) from the results of the ELISA test of estrogen hormone, an average of 709 ng/L was obtained and P3 (given ethanol extract of turmeric ( Curcuma longa Linn. syn. Curcuma domestica Val.) as much as 100 mg) from the results of the ELISA test of estrogen hormone, an average of 617 ng/L was obtained. There was no significant difference in the average estrogen levels between the control group and the treatment group. Conclusion: 96% turmeric ethanol extract at a dose of 100 mg/kg BB has the potential to be a natural antifertility agent by reducing ovarian weight in rats.