Development and Validation of a Spectrophotometric Method for the Estimation of Bictegravir using Chromogenic agents in Bulk and Pharmaceutical Dosage forms

A simple, precise, and accurate UV spectrophotometric method was developed and validated for the estimation of Bictegravir in tablet dosage forms. The method utilized methanol as a solvent, with λmax values determined to be 330 nm and 760 nm for reactions with the chromogenic reagents 2,4-dinitrophenylhydrazine (2,4-DNPH) and potassium ferrocyanide, respectively. Linearity was established within the concentration ranges of 10–25 µg/ml for 2,4-DNPH and 1–5 µg/ml for potassium ferrocyanide, both exhibiting R² values of 0.999.