Standardization And Quantification of Phytochemical Constituents, Metals and Minerals in Pun Purai Ennai, A Polyherbal Siddha Formulation

Background- Pun Purai Ennai, a traditional Siddha medicine, is notable for its potential healing properties. This study aimed to standardize and quantify its phytochemical constituents, focusing on its medicinal properties. Objectives- The primary objective was to assess the medicinal properties of Pun Purai Ennai by evaluating its phytochemical constituents, physical and chemical parameters, and potential therapeutic benefits. Methodology- The formulation, primarily composed of Indian Tulip Tree, was obtained from the Government Siddha Medical College in Chennai and includes several other components. The study evaluated the organoleptic characters, physical and chemical parameters, performed phytochemical screening, and quantified phytochemical constituents, minerals, and metals. Results- The evaluation revealed promising organoleptic characters and physical and chemical properties, indicating the formulation's purity. The phytochemical analysis revealed the presence of beneficial compounds like alkaloids, phenolics, flavonoids, and fatty acids, which are recognized for their antioxidant, anti-inflammatory, and antimicrobial effects. Quantification highlighted the therapeutic value of tannins and alkaloids. However, the presence of cyanogenic glycosides necessitates caution. The formulation also met safety standards for heavy metals and exhibited elements relevant to wound healing processes. Conclusion- These findings support the suitability of Pun Purai Ennai for wound healing, emphasizing the need for further research to explore its mechanisms of action and therapeutic efficacy.