Phytochemical Study And Evaluation Of The Antimicrobial Agonist Effect Of A. Sativum, S. Aromaticum And G. Kola Extracts

Allium sativum, Syzygium aromaticum and Garcinia kola are three substances highly prized for their antitussive properties. Recipe formulations involving the mixture of these substances are often reported but remain undocumented. This study was initiated with the aim of identifying any possible agonist effect that could exist between the three substances on strains of microbes responsible for some respiratory infections. During the conduct of the work, seven combinations were made in various proportions and then extracted with ethanol. They were all tested on six strains of microbes. The most active fraction was then the subject of phytochemical studies. It appears that the most active combination is the one containing 16.66% of A. sativum, 66.66% of S. aromaticum and 16.66% of G. kola (A1/S4/G1). It is the only one to have inhibited more germs (66.67%). It indicated the largest inhibition diameter of a value of 20 mm on clinical S. aureus. This fraction also presents the lowest Minimum inhibitory concentrations between 2.5 and 10 mg/mL and the lowest Minimum Bactericidal Concentration between 10 and 50 mg/ml. Phytochemical study of this fraction revealed the presence of reducing compounds (5.40%), alkaloids (2.14%), flavonoids (11.36%), phenolic compounds consisting of catechic and gallic tannins (9.70%), saponins (3.52%), terpenoids (0.98%), leucoanthocyanins, steroids, coumarins, mucilages, O-heterosides and free anthracenics. The intersection of the different results obtained indicates the agonist effect that exists between the three substances used and offers good prospects in the development of natural antibiotics against respiratory infections.