A Review On Mastering Bioanalytical Sample Preparation Techniques: Achieving Accurate Analysis

The Self-Double Emulsifying Drug Delivery System (SDEDDS) was a type of system where particles of one substance were dispersed within another. Simvastatin, a drug that lowered cholesterol, had a short half-life and only 5% bioavailability due to first-pass metabolism, making it a good candidate for SDEDDS. This study focused on improving Simvastatin's solubility and dissolution rate and various formulations of Simvastatin were prepared using SDEDDS, and all demonstrated quick emulsification times, high cloud points, and acceptable viscosity levels. The efficiency with which the drug was entrapped within the emulsions ranged from 82.12% to 97.75%, indicating a high level of effectiveness. A numerical optimization tool was employed to identify six optimal solutions, with the best combination consisting of 11 ml of olive oil, 52 ml of Span 80, and 10 ml of Tween 60, achieving a desirability score of 1.0. The optimized formulation exhibited an average particle size of 782.7 nm and a zeta potential of -46.4 mV, both of which are favorable for drug stability and absorption. Overall, this study demonstrated that SDEDDS could significantly improve the solubility, dissolution rate, and bioavailability of Simvastatin, potentially leading to enhanced therapeutic effects.