Development And Validation of UV Spectroscopic Method for Estimation of Calcium Pantothenate in Bulk and Pharmaceutical Dosage Form

The study aims to develop and validate a UV-visible spectrophotometric method for analyzing Calcium Pantothenate. The method uses a solvent system of methanol and water (1:1), chosen for its solubility and cost-effectiveness. A stock solution (1000 µg/ml) and subsequent dilutions were prepared for analysis. The absorption maxima (λmax) of Calcium Pantothenate was determined to be 212 nm. Validation parameters included linearity , precision , accuracy , specificity , robustness , and sensitivity. Linearity was confirmed over the concentration range of 5 to 25 µg/ml, with an R² value of 0.999. Precision studies both intra-day and inter-day demonstrated consistency with %RSD values below 2%. Accuracy was verified using the standard addition method with recoveries within 100±2%. The method’s specificity was confirmed by comparing the UV spectra of the tablet and standard solutions with no interference from excipients. The developed method is thus validated as reliable and accurate for the quantitative analysis of Calcium Pantothenate.