PHARMACOLOGICAL SCREENING AND PHYTOCHEMICAL EVALUATION OF ANTI-DIABETIC ACTIVITY OF DIOSPYROS PEREGRINA IN STREPTOZOTOCIN INDUCED DIABETIC RATS

Diabetes mellitus is a most common endocrine disorder, affecting more than 300 million people worldwide. For these therapies developed along the principles of allopathic are often limited in efficacy, Carry the risk of adverse effects, and are often too costly, especially for the developing world. To evaluate antidiabetic activity of Diospyros peregrine in streptozotocin (STZ) induced diabetes in albino rats albino rats were randomly divided into six groups (n=6). Diabetes was induced 16hrs intraperitoneal injection. After acute toxicity test, the Swiss albino mice were induced with streptozotocin (STZ) to get experimental diabetes animals. The fasting mean blood glucose level before and after treatment for normal, diabetic untreated and diabetic mice treated with aqueous and 70% ethanol extracts were performed. Data were statistically evaluated by using Statistical Package The acute oral toxicity studies of the extracts revealed no toxic effects up to the levels of 2000mg/kg b.wt. The aqueous and Methanolic extracts of 20 and 30mg/kg body weight of Diospyros peregrine was screened for the presence of hypoglycemic and antidiabetic activity. In this study diabetes was induced by a single IP dose streptozotocin (STZ) in 72hrs fasted rats. The FBGL was carried on 7th, 14th and 21st day and OGTT was measured on 8th, 15th and 22nd day. Glibeclamide was taken as the standard and the results are quite comparable with it. The studies were indicated that the leaves of Diospyros peregrine are effective in regeneration of insulin secreting β-cells and thus possess antidiabetic activity. The aqueous and Methanolic extracts showed significant effect in decreasing the Fasting blood Glucose level and oral glucose tolerance test of rats and it’s also showed good hypoglycemic activity in normal glycemic rats. The preliminary phytochemical analysis of the extracts of Diospyros peregrine revealed the presence of Alkaloids, Flavonoids, Steroids, Tannins, Anthraquinones, Terpenoids and Cardiac glycoside as the possible biologically active principles. Keywords: Diospyros peregrine, streptozotocin (STZ), Glibenclamide, FBGL and OGTT.