Isolation of Gram -ve Bacteria from the Root Extracts of Kalanchoe pinnata, Euphorbia tithymaloides and Murray koenigii

Gram -ve bacteria is highly virulent as compared to gram +ve microbes due to presence of outer membrane, lipoproteins and also due to the endotoxins they produce. Hence, their isolation and detailed studies on antimicrobial agents that can kill them is a challenging task. Earlier studies on Proteus spp. (P.mirabilis) has showed positive effect on growth of the plants like Lycopersicon esculentum Mill, commonly referred as Tomato plant and proven to be effective on plant development. In recent studies, we have isolated Gram -ve bacillus (Proteus spp.) from the root samples of Kalanchoe pinnata, Euphorbia tithymaloides and K. pneumoniae from Murray koenigii by plating serially diluted root extract samples on MacConkey agar and MacConkey Agar w/o CV, NaCl w/ 0.5% sodium taurocholate medium. The bacterial strains isolated were identified to be Gram -ve bacilli characterised using biochemical tests and Gram staining technique. The bacilli isolated from the rootlets of Kalanchoe pinnata and Euphorbia tithymaloides were found to be motile and identified as P.mirabilis and in case of Murray koenigii, the isolated bacilli were found to be non-motile and identified as Gram -ve K. pneumoniae.