A Research Article On UV Spectrophotometric Method Development And Validation Of Levofloxacin In Marketed Formulation Using Hydrotropy

The technique of ultraviolet-visible spectroscopy is one of the most frequently employed techniques in pharmaceutical analysis. Molecular absorption in ultraviolet & visible region of spectrum is depending on the electronic structure of molecules. Absorption of energy as quantized, resulting in the elevation of electrons from orbital in the ground state to higher orbital in the exited state. The term "hydrotropy" has been used to designate the increase in solubility of various substances due to the presence of large amounts of additives. Various hydrotropic agents such as sodium salicylate, sodium benzoate, urea, nicotinimide, sodium citrate and sodium acetate have been used to enhance the aqueous solubility of a large number of drugs. Method validation is the process of proving that an analytical method is acceptable for its intended purpose. For pharmaceutical methods, guidelines from the United States Pharmacopeia (USP), International Conference on Harmonization and the Food and Drug Administration provide a framework for performing such validations. Levofloxacin is a third-generation fluoroquinolone that is widely used in the treatment of mild-to-moderate respiratory and urinary tract infections due to sensitive organisms. Levofloxacin has been linked to rare instances of clinically apparent hepatic injury marked by a short latency period and a hepatocellular pattern of enzyme elevations.