A Review on Remdesivir by Pharmaceutical Analytical Methods [HPLC]

This review focuses on the pharmaceutical analytical profile of Remdesivir, a crucial antiviral drug used to treat viral infections like SARS-CoV-2. It emphasizes the drug's pharmacological importance and structural attributes, highlighting the critical need for precise analytical techniques to ensure its efficacy, stability, and safety. The review explores various methods for analyzing Remdesivir, each tailored to address specific aspects of its quality and performance. Non-aqueous titration assesses the drug’s solubility and stability in non-water solvents, while isothermal titration calorimetry (ITC) provides valuable insights into its interactions and thermodynamic properties. Electrochemical analysis offers sensitive and cost-effective evaluations of purity and stability. High-Performance Liquid Chromatography (HPLC) is noted for its reliability in routine quantification, with advanced techniques like LC-MS and UPLC-MS/MS ensuring the accurate detection of trace impurities and degradation products. Furthermore, spectrophotometric methods, including UV-Visible spectrophotometry and spectrofluorimetry, are highlighted for their simplicity and effectiveness in structural verification and quantitative analysis. Together, these techniques ensure Remdesivir's quality, safety, and therapeutic efficacy, supporting its critical role in pharmaceutical research and regulatory compliance.