PHYTOCHEMICAL SCREENING AND ANTI-ANXIETY ACTIVITY OF METHANOLIC AND CHLOROFORM EXTRACTS OF LANNEA COROMANDELICA LEAVES IN WISTER ALBINO MICE MODEL

Aim: This study aimed to evaluate the anti-anxiety activity of the Methanolic extract, and chloroform extract of Lannea coromandelica leaves in mice using an animal model. Methods: Psychopharmacological studies present antioxidants as a potential new strategy for the treatment of anxiety. The present paper deals with a preliminary phytochemical evaluation of the leaf Lannea coromandelica to establish anti-anxiety activity. Result: The study includes the preparation of different extracts and their phytochemical analysis and biological activity. Phytochemical studies revealed the presence of saponins, tannins, phenolics, terpenoids, and steroids. In the present investigation, Methanolic extract at 500 (µg/ml) showed 44.11% Scavenging effect & Chloroform extract at 500 (µg/ml) showed 51.47 %. These extracts showed moderate radical scavenging activity in the DPPH assay. This may be attributable to the high total phenolic and total flavonoid contents of the extracts. Elevated plus maze test was used to assess the psychomotor performance and emotional aspects of mice, treatment with Methanolic extract, and chloroform extract revealed anxiolytic activity, since the number of open arm entries and time spent in open arm parameters are the most delegated guide for anxiolytic activity Discussion: In our study, we demonstrated significant anti-anxiety activity with 500mg of both extracts compared to control group but has less activity when compared to that of diazepam but both were statistically significant. The average of basal activity scores in the control group after 30 and 60 minutes of administration of Methanolic, chloroform extracts of Lannea coromandelica leaves 500 mg/kg p.o. significantly reduced locomotor activity. Conclusion: It may be due to the CNS depressant property of the drug. The time spent by the animal in the central platform seems to be related to decision-making or risk assessment and the total arm entries is a measure reflecting changes in anxiety. Keywords: Lannea coromandelica, locomotor activity, anti-anxiety, DPPH assay