Экспериментальное применение модифицированного коллагенового кондуита при замещении дефекта седалищного нерва у крыс

Background. Numerous methods and technologies have been used in the development and modification of constructions for the repair of nerve defects. One of these techniques is obtaining biopolymer-based conductive conduits. To increase the efficiency of peripheral nerve repair, various fillers are used, such as extracellular matrix (ECM)-based hydrogels. Aim: To evaluate the reparative effect of an ECM-based hydrogel as a filler for a collagen nerve conduit in an in vivo model of peripheral nerve injury. Methods. The ECM-based hydrogel was obtained by alkaline hydrolysis of porcine dermis. Sciatic nerve defects were modeled in three groups of Wistar rats: group 1 (n=5), control, autografted defect; group 2 (n=5), defect reconstructed with a NeuraGen® collagen conduit (Integra, USA) with ECM-based hydrogel; group 3 (n=5), defect reconstructed with a NeuraGen® conduit without a filling. The reparative effect was evaluated by immunohistochemical staining to quantify the neurofilaments and motor fibers, and the extent of myelination in the proximal, medial, and distal fragments of the nerve. Results. The immunohistochemical evaluation of the implants showed elevated values of the expression of myelin basic protein (MBP), choline acetyltransferase (ChAT), and neurofilaments (NF) in the proximal segment of the nerve in all animal groups. In the autograft group, the expression values for the analyzed proteins were significantly greater than in the other groups. However, in group 2, the expression of the immunohistochemical markers was greater than in group 3 along the entire length of the reconstructed nerve, indicating a beneficial effect of the ECM-based hydrogel modification in the nerve conduit. Conclusion. The modification of the NeuraGen® collagen conduit with the ECM-based hydrogel enhanced the efficacy of the nerve tissue regeneration following the repair of peripheral nerve defects. The obtained hydrogel likely exerts a stimulatory effect on the growth of nerve fibers and enhances the migration and proliferation of Schwann cells, which underscores the need for further development and refinement of existing nerve conduits.