Spatiotemporal landscape of murine thymus reveals B cell-enriched inflammatory niche in thymus aging

Age-related thymic involution is closely associated with immunosenescence, inflammaging, and autoimmune disorders. However, the spatiotemporal features of developmental and aging thymus remain unknown. Here we constructed a spatiotemporal landscape of murine thymus from newborns to the aged using the self-developed spatial enhanced resolution omics-sequencing (Stereo-seq) and single-cell RNA sequencing (scRNA-seq).