Results of the Study of the IL 1β, IL 10, TNFα Gene Polymorphisms in Families with Helicobacter Pylori Infection

The aim is to determine the role of polymorphic loci of IL-1β, IL-10, TNF-α genes in families of patients with Helicobacter pylori-associated diseases. Materials and methods: Molecular genetic identification of H. pylori was carried out by PCR. Determination of cytokine gene polymorphisms (IL-1β (-31T/C), TNF-α (-308 G/A), IL-10 (-592C/A) and (+1082G/A) in 108 individuals – family members of individuals with chronic H. pylori-associated gastritis, was carried out by allele-specific PCR in real time. Results: H. pylori DNA was detected in 55.6% of patients' relatives, which is higher than the average prevalence in Russia (35.3%) and the Volga Federal District (33.0%). The distribution of gene polymorphisms of the studied cytokines in relatives of patients with and without H. pylori infection differed significantly. In H. pylori-infected family members, the T/T genotype and T allele of IL-1β (-31T/C), the C/C genotype of IL-10 (-592 C/A), and the G/G genotype of TNFα (-308 G/A) were significantly more frequently detected. No significant differences in IL-10 polymorphisms (+1082 G/A) were found in H. pylori positive and H. pylori negative individuals. Conclusions: IL 1β (-31T/C) T/T, IL 10 (-592 C/A) C/C, TNFα (-308 G/A) G/G gene polymorphisms increase the risk of H. pylori infection by more than 2 times. Carriers of such genotypes constitute a risk group for H. pylori-associated diseases, and in case of subclinical infection they are a source of infection and reinfection of relatives. Identification and treatment of infected family members is an important task in preventing the spread and recurrence of H. pylori infection.