Penicillin-binding protein occupancy dataset for 7 β-lactams, 2 β-lactamase inhibitors and combinations of 3 β-lactams/β-lactamase inhibitors in <i>Neisseria gonorrhoeae</i> strains ATCC 19424, ATCC 49226; clinical strains NG 3, NG 7, NG

<p>*CFM, 0.25–32 mg/L; CTX, 0.125–16 mg/L; CRO, 0.016–2 mg/L; CAZ, 0.25–32 mg/L; TOL, 1–128 mg/L; PIP, 0.016–2 mg/L; AVI, 2–256 mg/L; TZ, 1–128 mg/L; CAZ/AVI, 0.25–32 mg/L (avibactam 4 mg/L fixed concentration); TOL/TZ, 1–128 mg/L (tazobactam 4 mg/L fixed concentration). PBP-binding assay gels for N. gonorrhoeae strains ATCC 19424, ATCC 49226; clinical strains NG 3, NG 7, NG 12, NG 14, NG 19, NG 20, NG 21 and NG 22; WHO reference strains NCTC 13820 (WHO X), NCTC 13821 (WHO Y) and NCTC 13822 (WHO Z). 1 Drugs tested were ertapenem (ETP), cefixime (CFM), cefotaxime (CTX), ceftriaxone (CRO), ceftazidime (CAZ), ceftolozane (TOL), piperacillin (PIP), avibactam (AVI), tazobactam (TZ), ceftazidime/avibactam (CAZ/AVI), ceftolozane/tazobactam (TOL/TZ), and piperacillin/tazobactam (PIP/TZ). The antibiotic-bound PBP-containing membrane preparations were label with 25 μM Bocillin FLTM. Labeled PBPs were separated by SDS-PAGE and detected using a fluorimager. The global range of concentrations tested was 0.001 to 512 mg/L.</p> <p>(PDF)</p>