DENV infection of polyclonal B cells by BCR/DENV cross-linking.

<p><b>A)</b> Schematic representation of DENV/Ig/BCR crosslinking assay. Created in BioRender. <b>B)</b> Representative flow cytometry plot showing the frequency of DENV-4 infected Raji cells under the indicated culture conditions. Detection of DENV-infected cells was performed by staining fixed/permeabilized cells with a FITC-conjugated 4G2 antibody. <b>C)</b> Quantification of DENV-4 infected Raji cells under the indicated culture conditions. <b>D)</b> Gating scheme for B cell infection analysis utilizing the DENV/Ig/BCR crosslinking assay.</p> <p>(PDF)</p>