Binding of recombinant OmpR to the promoter region of VK055_3347.

<p><b>(A)</b> Diagrams of the promoter region of the VK055_3347 locus (not drawn to scale). The black box represents a putative OmpR binding sequence. Nucleotide numberings are relative to the VK055_3347 ATG codon, respectively. The relative position and length of the DNA fragment used in EMSA are shown. <b>(B)</b> EMSA results of rOmpR. The P<sub>VK055_3347</sub> DNA fragment was mixed with increasing concentrations of phosphorylated rOmpR at 0, 0.3, 0.7, and 1 μg (Lanes 1–4). The P<sub>gcvT</sub> DNA fragment was used as a negative control, mixing with phosphorylated rOmpR at 0, 1 and 4 μg (Lanes 1–3). DNA bands were detected by Gelred staining. The positions of DNA fragments that had not shifted were labeled.</p> <p>(PDF)</p>