Example outcomes for (A, B) 10F4R and (C, D) 2F8R assays in RT-QuIC ED experiments.

<p>(A), (C) Observed number of positive wells in the RT-QuIC assay (black circles) and expected number of wells with seeds as a function of sample dilution for the most likely value of the log<sub>10</sub> SD50/mg estimated via the SK (green solid), RM (orange dashed), Poisson (purple dotted), or midSIN (blue dash-dot) methods (indicated in this order above the graph); the short vertical lines indicate the sample dilution at which ED wells would be expected to receive 1 SD50. (B), (D) Posterior likelihood distribution for the log<sub>10</sub> SD50/mg estimated by midSIN, with the vertical lines corresponding to estimates for each method.</p> <p>(DOCX)</p>