LANA cannot recruit MeCP2 even in HCT-DKO cells.

HCT116 (A) and DKO (DNMT3B-/- and DNMT1 -/-) HCT116 cells (B) were transfected with dCas9-SunTag, scFv-LANA, and sgTelomere as illustrated on the left of the images. An immunofluorescence assay was performed to detect MeCP2 (green) and LANA (red). The nucleus was stained with DAPI. Scale bar = 5 μm. Images are representatives of at least two independent experiments. The plots of the red, green, and blue pixel intensities along the white arrow (in the middle panels) are presented. (<b>C</b>) Pearson’s correlation coefficient was determined by ImageJ (JACoP Plugin) for 15 cells in each treatment and presented as box and whiskers (min to max). Two-tailed t tests were performed (*, P ≤ 0.05; **, P ≤ 0.01; ***; P ≤ 0.001). (PDF)