Enzymatic activity of lysozyme and control of protein loading.

(A) Enzymatic activity of recombinant <i>N</i><i>aegleria</i> <i>fowleri</i> lysozyme (blue line) and chicken egg white lysozyme (red line). A decrease in absorbance at 450 nm over time is associated with lysozyme enzymatic activity. The black line represents a blank sample without enzyme addition. (B) Control of protein loading for the immunoblot of <i>N. fowleri</i> lysozyme in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1012995#ppat.1012995.g004" target="_blank">Fig 4</a>, stained by Ponceau S. ax.: total lysate from axenically cultured <i>N. fowleri</i>, g-: <i>N. fowleri</i> co-cultured with <i>Klebsiella aerogenes</i>, g+: <i>N. fowleri</i> co-cultured with <i>Micrococcus lysodeikticus</i>, fib.: <i>N. fowleri</i> co-cultured with HT1080 fibrosarcoma cells. (PDF)