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<div> This study investigated the parasitic selection preferences of female <i>Trichogramma ostriniae</i> on egg cards of different colors, considering the effects of inoculation time, generations, and learning experiences on wasp parasitism. The preferences of female <i>T</i>. <i>ostriniae</i> for oviposition were assessed across egg cards of different colors. Subsequent experiments identified the egg card background color most preferred by <i>T</i>. <i>ostriniae</i> and examined the influence of inoculation time, generations, and learning experiences on parasitism rates. The findings revealed a significant preference for <i>T</i>. <i>ostriniae</i> for yellow egg cards compared to other colors, with the highest parasitism rate observed on yellow and the lowest on red. Inoculation time had a significant effect on the parasitism of <i>T</i>. <i>ostriniae</i> on yellow egg cards, with the highest parasitism observed at 16h post-inoculation and a noticeable negative linear correlation with increasing inoculation time up to 48h (F = 8.73, P<0.05). The number of generations did not significantly affect the parasitism rate of <i>T</i>. <i>ostriniae</i> on yellow egg cards, nor did the learning experiences show a significant difference in the relative parasitism of <i>T</i>. <i>ostriniae</i> on yellow egg cards among the learning experiences. However, T. ostriniae had a significantly higher parasitization rate on yellow egg cards compared to white egg cards, regardless of generation or learning experience. Those results showed that female <i>T</i>. <i>ostriniae</i> strongly prefers yellow. The highest parasitism of female <i>T</i>. <i>ostriniae</i> on yellow egg cards was observed at 16h post-inoculation. </div>