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<b>S1 Fig.</b> The three-dimensional structure of HEK239/17 and A549 cells infected with DENV, JEV, and ZIKV at 12 and 24 hpi. Confocal microscopy determined the colocalization between FASN and NS3/dsRNA of (A) DENV (B) ZIKV (C) JEV. The images were taken in approximately 30 stacks, with 63X magnification and 1.5X Zoom. Each color represents different fluorochrome staining proteins. For staining of FASN and NS3, FASN is represented in green (AlexaTM Fluor 488), and NS3 is represented in red (AlexaTM Fluor 647). For staining of FASN and dsRNA, FASN is represented in red (AlexaTM Fluor 647), and NS3 is represented in green (AlexaTM Fluor 488). The nucleus is represented in blue (DAPI). The percent colocalization was analyzed by Imaris program (version 9.9.0) shown in the right panels. <b>S1 Table.</b> Primer sequences for constructing NS3 and FASN. <b>uncropped western blots.</b> (PDF)

Publication Details

Journal
Figshare
Published
2025-03-25
DOI
https://doi.org/10.1371/journal.pone.0319207.s001
Primary Topic
Virus-based gene therapy research
Type
article
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0.00
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Virus-based gene therapy research
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Abstract

<b>S1 Fig.</b> The three-dimensional structure of HEK239/17 and A549 cells infected with DENV, JEV, and ZIKV at 12 and 24 hpi. Confocal microscopy determined the colocalization between FASN and NS3/dsRNA of (A) DENV (B) ZIKV (C) JEV. The images were taken in approximately 30 stacks, with 63X magnification and 1.5X Zoom. Each color represents different fluorochrome staining proteins. For staining of FASN and NS3, FASN is represented in green (AlexaTM Fluor 488), and NS3 is represented in red (AlexaTM Fluor 647). For staining of FASN and dsRNA, FASN is represented in red (AlexaTM Fluor 647), and NS3 is represented in green (AlexaTM Fluor 488). The nucleus is represented in blue (DAPI). The percent colocalization was analyzed by Imaris program (version 9.9.0) shown in the right panels. <b>S1 Table.</b> Primer sequences for constructing NS3 and FASN. <b>uncropped western blots.</b> (PDF)

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Virus-based gene therapy research
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