Epitope profiling and pan-sarbecovirus RBD binding activity for C68 mAbs.

<p><b>(A)</b> Complete escape maps of antibody-binding escape mutations for C68 mAbs using a yeast-displayed SARS-CoV-2 RBD deep mutational scanning system (Omicron BA.2 RBD). Line plots (left panel) show escape at each site in RBD. Residue colors are assigned based on effect these mutations have on RBD expression (with yellow indicating mutations deleterious for RBD expression). The height of letters in the logo plots indicate level of escape by that amino acid at that site. Logo plot residue numbering is based on SARS-CoV-2 Omicron BA.2 <b>(B)</b> Pan-sarbecovirus RBD yeast display to assess C68 mAbs sarbecovirus RBD binding breadth. EC50 value represents the geometric mean across the independent barcodes for corresponding sarbecovirus RBD.</p> <p>(PDF)</p>