Primer sets and quantitative real-time PCR conditions for quantification of copy number and measurement of methylation level of <i>LINE-1</i> and <i>Alu</i>.

<p>The methylation-dependent-specific PCR (MSP) primers (Me-) are designed from the consensus sequences of <i>LINE-1</i> and <i>Alu</i> (S1 Fig). All non-CpG cytosines were replaced by ‘t” in the forward primers and by “a” in the reverse ones.</p> <p>(PDF)</p>

Publication Details

Journal
Figshare
Published
2024-12-30
DOI
https://doi.org/10.1371/journal.pone.0316394.s001
Primary Topic
Molecular Biology Techniques and Applications
Type
article
Field-Weighted Citation Impact
0.00
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article

Primer sets and quantitative real-time PCR conditions for quantification of copy number and measurement of methylation level of LINE-1 and Alu.

Figshare
Molecular Biology Techniques and Applications
article

Primer sets and quantitative real-time PCR conditions for quantification of copy number and measurement of methylation level of LINE-1 and Alu.

article en

Abstract

<p>The methylation-dependent-specific PCR (MSP) primers (Me-) are designed from the consensus sequences of <i>LINE-1</i> and <i>Alu</i> (S1 Fig). All non-CpG cytosines were replaced by ‘t” in the forward primers and by “a” in the reverse ones.</p> <p>(PDF)</p>

Figshare
Openalex Percentile: Top 76%
Molecular Biology Techniques and Applications
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