Overexpression of the high affinity copper transporter <i>ctr3</i> and the metalloreductase <i>fre9</i> rescues restores virulence of the <i>mac1</i>Δ mutant on the animal host <i>Galleria mellonella</i&gt

<p><b>(A, B)</b> Kaplan-Meier plot showing survival of groups of 15 <i>G</i>. <i>mellonella</i> larvae after injection into the hemocoel of 1.6x10<sup>5</sup> microconidia of the indicated <i>F</i>. <i>oxysporum</i> strains or phosphate-buffered saline (PBS) as a negative control. Insects were maintained at 30°C. <i>p</i>-value: ****<0.0001 versus the wt according to Log-rank (Mantel-Cox) test. Data shown are from one representative experiment. Experiments were performed at least two times with similar results.</p> <p>(PDF)</p>