Effect of PKCθ inhibition in CD8<sup>+</sup> T cells on phosphorylation of TBK1.

<p>TBKBP1-overexpressing CD8<sup>+</sup> T cells and EV-transduced controls were generated, and successfully transduced CD8<sup>+</sup>mCherry<sup>+</sup> T cells were sorted by flow cytometry, serum-starved overnight, preincubated with the PKCθ inhibitor or DMSO as control, and subsequently short-term stimulated. Samples from EV-transduced or TBKBP1-overexpressing CD8<sup>+</sup> T cells were subjected to immunoblotting to determine the expression of TBKBP1, pTBK1, and total TBK1. The analysis of α-Tubulin expression served as loading control. Data from two independent donors are depicted.</p> <p>(PDF)</p>