Original western blot images with membranes stained with Ponceau S.

<p>The upper half of the membrane was used to detect pS295 or PSD-95, whereas the lower half was used for pJnk1, Jnk1, pGsk3β, or Gsk3β. Two membranes connected by a dashed line are originally a single membrane at the blotting step. Transferred proteins on the membranes were monitored with Ponceau S staining after the signal detection using chemiluminescence. Some Ponceau-stained membranes (unused half of the membrane) are included as references for monitoring loaded proteins. In the lanes labeled with "E", protein extracts for filling empty lanes are loaded.</p> <p>(PDF)</p>