RNA integrity number equivalents (RIN) for RNA samples obtained from hypothalamus of experimental animals.

<p>The RNA integrity was assessed by the Genomics Core Facility of the Faculty of Medicine at Ulm University via automated gel electrophoresis (Agilent 4200 TapeStation System, Agilent Technologies Deutschland GmbH, Waldbronn, Germany). Table includes also 28S to 18S ratio and RNA concentration in the sample measured in NanoDrop (NanoDrop). The RIN numbers was assessed later, therefore we measured RNA concentration once again before RIN measurements. The concentration of RNA measured before qPCR analysis and concentration of RNA measured before RIN analysis was 0.99. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0309591#pone.0309591.s002" target="_blank">S1 Fig</a>. Therefore we presented the first measured RNA concentration in this table.</p> <p>(DOCX)</p>

Publication Details

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Figshare
Published
2024-10-25
DOI
https://doi.org/10.1371/journal.pone.0309591.s001
Primary Topic
Molecular Biology Techniques and Applications
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article

RNA integrity number equivalents (RIN) for RNA samples obtained from hypothalamus of experimental animals.

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Molecular Biology Techniques and Applications
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RNA integrity number equivalents (RIN) for RNA samples obtained from hypothalamus of experimental animals.

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Abstract

<p>The RNA integrity was assessed by the Genomics Core Facility of the Faculty of Medicine at Ulm University via automated gel electrophoresis (Agilent 4200 TapeStation System, Agilent Technologies Deutschland GmbH, Waldbronn, Germany). Table includes also 28S to 18S ratio and RNA concentration in the sample measured in NanoDrop (NanoDrop). The RIN numbers was assessed later, therefore we measured RNA concentration once again before RIN measurements. The concentration of RNA measured before qPCR analysis and concentration of RNA measured before RIN analysis was 0.99. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0309591#pone.0309591.s002" target="_blank">S1 Fig</a>. Therefore we presented the first measured RNA concentration in this table.</p> <p>(DOCX)</p>

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Molecular Biology Techniques and Applications
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RNA integrity number equivalents (RIN) for RNA samples obtained from hypothalamus of experimental animals. · Figshare (2024) | TGRS Research Map | TGRS