S1 Fig -

<p>Examples of Plaque-Forming Units (PFU) assays of mosquito samples (A, bodies and heads; B, saliva) from the F0_SM population infected with the DENV-1 strain. For bodies and heads, tenfold serial dilutions (-1 to -3) from each specimen were performed. Monolayers of Vero cells in 48 -well plates (for bodies and heards) or 12_well plates (for saliva) were infected, incubated for 2 h at 37 o C with 5% CO2 incubator. After 5 days of incubation, plates were fixed and stained with crystal violet. Number 1, 2, 5, 9, 24, 35 and 37 correspond to samples. Number corresponding to positive saliva (5, 9 and 24) are in yellow.</p> <p>(DOC)</p>