Average expression of multiple <i>e2f8</i> and <i>stat5a</i> transcripts in <i>Tg(isl2b:GFP)</i> and DN-Jun(+) fish.

RT-qPCR of putative Jun target genes’ showing average expression during regeneration in <i>Tg(isl2b:GFP)</i> X <i>Tg(isl2b:GFP),</i> (green) and Jun knockdown <i>Tg(isl2b:GFP)</i> X <i>Tg(mke15Tg)</i> DN-Jun(+), (orange) fish. (<b>A–B</b>) Average fold change of three individual replicates are plotted for each condition and timepoint. Each timepoint required n = 90 retinas (30 retinas per replicate for 3 replicates) for both control and knockdown experiments. <i>e2f8</i> forward 5’-TCTTCGTGAAACCCATGTCA-3’ <i>e2f8</i> reverse 5’-GACCGCCTTTAGGTGTGGTA-3’; <i>stat5a</i> forward 5’-TGACCCGAGAAGCTAACACC-3’ <i>stat5a</i> reverse 5’-GTATGTCCAGTCCTCCCT-3’. (<b>C–E</b>) Individual replicates for averages shown in A and B. <i>Tg(isl2b:GFP)</i> are shown in C and D. DN-Jun(+) are shown in E and F. (DOCX)