Screen for integration of PX459 GOLGA8 guide RNA plasmid.

<p>Plasmid map of PX459 containing guide RNA targeting GOLGA8 repeat sequences. Primers for individual amplicons are indicated on the map. The plasmid was used as a positive control for PCR reactions 1 through 3. An additional amplicon for the <i>TBX5</i> promoter was used as a positive control for genomic DNA for each PCR reaction (number 4). For each of the lines, including the parental H9 line, the <i>TBX5</i> amplicon amplified. The PX459 amplicons were detected from purified plasmid DNA but failed to amplify from any genomic DNA sample from edited clones.</p> <p>(PDF)</p>