Generation of a <i>Fusarium oxysporum mac1</i><sup>clover</sup> strain.

<p><b>(A)</b> Physical map of the <i>F</i>. <i>oxysporum mac1</i><sup>clover</sup> DNA construct. Relative positions of PCR primers are indicated. <b>(B)</b> Agarose gel electrophoresis of PCR products obtained using primer pairs Mac1-qPCR-F and EYFPrev with genomic DNA extracted from the indicated transformants. M, molecular size markers. <b>(C)</b> Colony phenotypes of the indicated strains grown for 2 d at 28°C on MM+TE<sup>-Cu</sup> (20 mM NaNO<sub>3</sub>) without any copper supply. Scale bar, 0.5 cm. <b>(D)</b> Transcript levels of the indicated genes in the indicated strains transferred for 6 h to MM-TE<sup>-Cu</sup> with (+Cu) or without (-Cu) 100 μM CuSO<sub>4</sub> were measured by real-time RT-qPCR and expressed relative to those of the wt strain in -Cu. Bars represent standard deviations (<i>n</i> = 3, biological replicates). <i>p</i>-vaules: ns>0.05, *<0.05, **<0.01 versus -Cu, in each strain, according to two-tailed unpaired Student’s <i>t</i> test.</p> <p>(PDF)</p>