S2 Fig -

<p>(A) Relative stability and replication ability of transfected replicon RNA in cells. Replicon RNA transcripts were transfected into cells, washed after 15 minutes and harvested after either 15 minutes or 6 hours post-transfection (equivalent to the harvest timepoint for the trans-encapsidation assay) in the presence or absence of 3 mM GuHCl. After extracting the RNA, relative RNA yields were assessed by RT-qPCR based on the Ct values. The experiment was performed in triplicate, with the means and standard errors shown here. No significant differences were seen between the constructs with PK deletions and the equivalent sample for the wt replicon (P < 0.005), nor were marked differences seen in the presence or absence of GuHCl for each construct. The dotted line represents the cut-off point. (B) RNA abundance values, including the Mean and Standard Error data, for S1A. Values were calculated based on the ct values (assuming 100% polymerase efficiency) and multiplied by 10^12 to improve the scale for ease of interpretation.</p> <p>(PDF)</p>