Original images of diagnostic PCRs and Southern blot image supporting Fig 2.

<p>PCR analysis of eight transformed strains was performed using primer pairs (A) 2245/2246 and (B) 2247/2248 to confirm homologous integration of the expression cassette at the <i>niaD</i> gene locus. (C) Southern blot analysis of eight strains transformed with <i>Agrobacterium sp</i>. AGL-1_AO11 was performed using <i>Hind</i>III-digested DNA from transformants and wild type (WT), with the entire T-DNA fragment as a probe. The Thermo Scientific GeneRuler 1 kb DNA Ladder was used as a marker in all gels.</p> <p>(PDF)</p>