Phenotypic characterisation of T(CMV) cells.

<p>PBMCs from healthy CMV-seropositive donors were pre-enriched for CD8<sup>+</sup> T cells and subsequently stimulated with the CMVpp65 overlapping peptide pool. Next, IFN-γ-secreting cells were detected using the IFN-γ Secretion Assay/Detection Kit. The phenotype of IFN-γ-secreting T(CMV) cells was determined using flow cytometry. <b>(A)</b> Representative flow cytometry plots show the identification of T(CMV) cells (left) and the phenotypic characterisation via CD45RA and CD62L expression (right). Numbers indicate frequencies in gates or quadrants. <b>(B)</b> The bar plot shows the frequencies of T<sub>N</sub> (CD45RA<sup>+</sup>CD62L<sup>+</sup>), T<sub>CM</sub> (CD45RA<sup>-</sup>CD62L<sup>+</sup>), T<sub>EM</sub> (CD45RA<sup>-</sup>CD62L<sup>-</sup>) and T<sub>EMRA</sub> cells (CD45RA<sup>+</sup>CD62L<sup>-</sup>) within T(CMV) cells from 4 donors. Black dots indicate frequencies from individual donors and grey bar mean values with SD.</p> <p>(PDF)</p>